Description

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4⁺ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Barclay AN. 1981. Immunology 42:593. (IHC)
2. Wallgren AC, et al. 1995. Transplantation 60:594. (IHC)
3. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB)
4. Mason DW, et al. 1983. Immunol. Rev. 74:57.
5. Hirji N, et al. 1997. J. Immunol. 158:1833. (FA)
6. Mitnacht R,, et al. 1998. J. Immunol. 160:700. (WB)