Purified anti-CD49C (Integrin α3)

Antibodies Single
Sony
P1B5
Flow Cytometry,Immunohistochemistry,Other
Mouse IgG1, κ
Human
The P1B5 monoclonal antibody was generated against human HT1080 cells.
4926010
$285.00

Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For flow cytometric staining, the suggested use of this reagent is between 1.0 - 10 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

References

  1. Wayner EA and Hoffstrom BG. 2007. Methods. Enzymol. 426:117.
  2. Wayner EA, et al. 1993. J Cell Biol. 121:1141. (IP)
  3. Carter WG, et al. 1990. J Cell Biol. 110:1387-404 (IP)
  4. Wayner EA and Carter WG. 1987. J Cell Biol. 105:1873. (IP)