PE/Cy5 anti-mouse CD5

Antibodies Single
Sony
53-7.3
Flow Cytometry
Rat IgG2a, κ
Mouse
Mouse thymus or spleen
1103045
$82.00

Description

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
2. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
3. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.