PE anti-PLCγ1 Phospho (Tyr783)

Antibodies Single
Sony
A17025A
Intracellular Flow Cytometry
Mouse IgG1, κ
Human
Synthetic peptide from human plcy1 phosphorylated at Try783 emulsified in CFA
3662015
$122.00

Description

Phospholipase C (PLC) enzymes cleave the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) into second messenger molecules inositol 1,4,5-triposphate (IP3) and diacylglycerol (DAG) in response to extracellular stimuli. IP3 causes the release of calcium from the endoplasmic reticulum to begin an intracellular signaling cascade, while DAG activates Protein Kinase C.

There are four families of PLCs. PLC gamma (PLCγ) uniquely contains SRC domains SH2 and SH3. Receptor tyrosine kinases interact with these SRC domains to enzymatically activate PLCγ1 via phosphorylation at Tyr 783. PLCγ1 is essential for cell proliferation, differentiation, and motility.  Overexpression or activation of PLCγ1 has been implicated in breast and prostate cancers, as well as cancer cell invasion.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.