Pacific Blue™ anti-mouse CD11a/CD18 (LFA-1)
Description
Lymphocyte function-associated antigen 1 or LFA-1 is a heterodimer composed of two members of the integrin family, αL (CD11a) and β2 (CD18). LFA-1 is expressed primarily on lymphocytes, monocytes/macrophages, and granulocytes. It is involved in cell adhesion and costimulation processes. The ligands for LFA-1 are ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
References
- Pont S, et al. 1986. J. Immunol. 136:3750. (FC)