Brilliant Violet 421™ anti-mouse CD1d (CD1.1, Ly-38)

Antibodies Single
Sony
1B1
Flow Cytometry
Rat IgG2b, κ
Mouse
Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma
50 µg
1217635
$267.00

Description

CD1d, known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e). But CD1d is the only CD1 molecule has been found in mouse. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. It has been shown that PKCδ is a critical regulator of CD1d-mediated antigen presentation.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.



This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

  1. Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block)
  2. Brozovic S, et al. 2004. Nat. Med. 10:535.
  3. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block)
  4. Jiang J, et al. 2012. PLoS One. 7:47487. PubMed