Description

The AF6-88.5 antibody reacts with H-2K^b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K^b haplotype. H-2K^b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

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6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed