Description

CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. DiSalvo J, et al. 1995. J Biol Chem. 270:7717.
2. McColl BK, et al. 2003. J Exp Med. 198:863.
3. Albuquerque RJ, et al. 2009. Nat Med. 15:1023.
4. Guangqi E, et al. 2012. J Biol Chem. 287:3029.
5. Koch S, and Claesson-Welsh. 2012. Cold Spring Harb Perspect Med. 2:a006502.
6. Munaut C, et al. 2012. Plos One. 7:e33475.
7. Wehland M, et al. 2013. Int J Mol Sci. 14:9338.