APC anti-rat CD47

Antibodies Single
Sony
OX-101
Flow Cytometry
Mouse IgG1, κ
Rat
Mouse CXCR3-transfectants
1620570
$237.00

Description

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment, and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. The significance of this molecule is recently drawing increasing attention. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

  1. Vernon-Wilson, et al. 2000. Eur. J. Immunol. 30:2130.
  2. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
  3. Mawby WJ, et al. 1994. Biochem. J. 304:525.