APC anti-FITC

Antibodies Single
Sony
FIT-22
Flow Cytometry
Mouse IgG1, λ
FITC conjugated mouse IgG.
2641545
$52.00

Description

The FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.