PE/Dazzle™ 594 anti-human CD137L (4-1BB Ligand)
Description
4-1BB ligand, also known as CDw137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
References
1. Akiba H, et al. 2000. J. Exp. Med. 191:375.
2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488.
3. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.