PE anti-RPS6 Phospho (Ser235/Ser236)

Antibodies Single
Sony
A17020B
Intracellular Flow Cytometry
Mouse IgG1, κ
Human,Mouse
Synthetic peptide from human RPS6 phosphorylated at Serines 235 and 236
3643015

Description

Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the  7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabeteic mouse models.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

  1. Jefferies HB, et al. 1997. EMBO J. 16:3693.
  2. Ruvinsky I, et al. 2005. Genes. Dev.19:2199.
  3. Chumacher AM, et al. 2006. Biochemistry. 45:13614
  4. Roux PP,