APC/Cy7 anti-human CD25

Antibodies Single
Sony
BC96
Flow Cytometry
Mouse IgG1, κ
Human
2113065
$151.00

Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (IF) PubMed
4. Cornwell WD, et al. 2013. J Neuroimmunol. 265:43. PubMed