Pacific Blue™ anti-human CD294 (CRTH2)

Antibodies Single
Sony
BM16
Flow Cytometry
Rat IgG2a, κ
Human
Rat cell line TART/B19-12.10 transfected with human CRTH2
2350645
$116.00

Description

CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711.
2. Monneret G, et al. 2001. Blood 98:1942.
3. Xue L, et al. 2009. J. Immunol. 182:7580.
4. Schratl P, et al. 2007. J. Immunol. 179:4792.