Pacific Blue™ anti-mouse TCR Vβ5.1, 5.2

Antibodies Single
Sony
MR9-4
Flow Cytometry
Mouse IgG1, κ
Mouse
Murine T cell hybridoma 2HB51.8
1297580
$226.00

Description

Vβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. 

References

1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171.
2. Sim GK and Augustin AA. 1985. Cell 42:89.
3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.