Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β₁, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Wayner EA, Hoffstrom BG. 2007. Meths Enzymol In: Integrins. 426:117. PubMed 
2. Wang HQ, et al. 2007. Eur J Cell Biol. 86(1):51. (Block) PubMed 
3. Da Silva MS, et al. 2003. Surgery. 134(2):164. (Block) PubMed 
4. Blaschke R, et al. 2002. Biochem Biophys Res Commun. 296(4):890. (Block) PubMed 
5. Dittel BN, et al. 1993. Blood. 81:2272. (FC) PubMed