Human TruStain FcX™ (Fc Receptor Blocking Solution)



Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells. The cells with FcR expression sometimes give false positive or false negative results of immunofluorescent staining due to the FcRs-mediated Ig Fc binding. Human TruStain FcX™ is specially formulated for blocking the FcR-involved unwanted staining without interfering with antibody-mediated specific staining of human cells. Human TruStain FcX™ is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies.

Recommended Usage

It is recommended to add 5 microL of Human TruStain FcX™ per million cells in 100 microL staining volume, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.


1. de la Cruz J, et al. 2011. J. Immunol. 186:2282. PubMed
2. Luo L, et al. 2011. Exp Mol Pathol. 91:596. PubMed
3. Szerlip NJ, et al. 2012. PNAS. 109:3041. PubMed
4. Bromley SK, et al. 2013. J. Immunol. 190:970. PubMed
5. Trujilo G, et al. 2013. J. Immunol. 191:848. PubMed
6. Edin S, et al. 2013. PLoS One. 18:74982. PubMed
7. Euzebio Alves VT, et al. 2013. Infect Immun. 81:4399. PubMed
8. Borg ZD, et al. 2014. J. Immunol. 192:189. PubMed
9. Klingenberg R, et al. 2014. Eur Heart J. PubMed
10. Lines JL, et al. 2014. Cancer Res. 74:1924. PubMed
11. Decker D, et al. 2014. Lupus. 23:802. PubMed
12. Jitschin R, et al. 2014. Blood. 123:2663. PubMed
13. Anderson ME Jr, et al. 2014. Otolaryngol Head Neck Surg. 150:880. PubMed
14. Giurisato E, et al. 2014. Mol Biol Cell. 25:1769. PubMed
15. Shi J, et al. 2014. Blood. 123:4015. PubMed
16. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
17. Fujisawa D, et al. 2014. J Allergy Clin Immunol. 134:622. PubMed
18. Bhardwaj N, et al. 2014. J Virol. 88:11108. PubMed
19. Lieber S, et al. 2014. Mol Pharmacol. 87:162. PubMed
20. Olagnier D, et al. 2014. PLoS Pathog. 10:1004566. PubMed
21. Punyadee N, et al. 2015. J Virol. 89:1587. PubMed
22. Vuong TT, et al. 2015. J Histochem Cytochem. 63:280. PubMed
23. Klingenberg R, et al. 2015. Eur Heart J. 36:1041. PubMed
24. Wilkinson-Ryan I, et al. 2015. PLoS One.10:125851. P