PE anti-mouse/human Helios

Antibodies Single
Sony
22F6
Flow Cytometry
Hamster IgG
Human,Mouse
Helios peptide (aa 51-107)
1286030
$203.00

Description

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry . It is recommended that the reagent be titrated for optimal performance for each application.
Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed