Alexa Fluor® 647 anti-HA.11 Epitope Tag
Description
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 0.1-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
References
1. Helliwell SB, et al. 2001. J. Cell. Biol. 153:649. (WB)
2. Bennett BD, et al. 2000. J. Biol. Chem. 275:37712. (IF, IP, WB)
3. Collier JH, et al. 2006. Proc. Acad. Sci. USA 103:2021. (IF)
4. Datler C, et al. 2013. Biochim. Biophys. Acta. 12:2844. (FC)
5. Kim JY, et al. 2003. J. Neurosci. 23:5561. (IP, WB)