PerCP anti-human TNF-α

Antibodies Single
Sony
MAb11
Flow Cytometry
Mouse IgG1, κ
Human
<I>E. coli</I>-expressed, recombinant human TNF-α
3114615
$151.00

Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

1. Rathjen D, et al. 1991. Mol. Immunol. 28:79. (Neut)
2. Danis V, et al. 1991. Clin. Exp. Immunol. 85:143. (Neut)
3. Enr quez J, et al. 2002. Adv. Perit. Dial. 18:177. (ICFC)
4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
5. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
7. Andersson U, et al. 2000. J. Exp. Med. 192:565. (ICC)
8. Moormann AM, et al. 1999. J. Infect. Dis. 180:1987. (IHC)
9. Zhao XJ, et al. 2003. J. Immunol. 170:2923. (IF)
10. Rieger R, et al. 2009. Cancer Gene Ther. 1:53-64. (FC)