Brilliant Violet 711™ anti-human IL-2

Antibodies Single
Sony
MQ1-17H12
Flow Cytometry
Rat IgG2a, κ
Human
<I>E. coli</I> - expressed recombinant human IL-2
3101730
$468.00

Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)