Purified anti-human CD99

Antibodies Single
Sony
3B2/TA8
Flow Cytometry
Mouse IgG1, κ
Human
Human thymus
100 µg
2456510
$121.00

Description

CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin β 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 microg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Waclavicek M, et al. 1998. J. Immunol. 161:4671.
2. Pickl W, et al. 2001. J. Virol. 75:7175.