Purified anti-human CD45

Antibodies Single
Sony
2D1
Immunofluorescence
Mouse IgG1, κ
Human
Human PBMC
100 µg
2442510
$104.00

Description

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
4. Lee MS, et al. 2004. Int. Immunol. 16:1109.