Alexa Fluor® 647 anti-BrdU
Description
BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
References
1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
3. Godebu E, et al. 2008. J. Immunol. 181:1798.
4. Waskow C, et al. 2008. Nat. Immunol. 9:676.