Pacific Blue™ anti-human CD5

Antibodies Single
Sony
L17F12
Flow Cytometry
Mouse IgG2a, κ
Human
Human T-acute lymphoblastic leukemia (ALL) cells
2420115
$121.00

Description

CD5, also known as Leu-1, Ly-1 and T1, is a 67 kD single chain type I glycoprotein that is a member of the scavenger receptor superfamily. CD5 is expressed on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-Cells), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions. One of its ligands is CD72.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. McAlister MS, et al. 1998. Protein Eng. 11:847. (IP)
2. Butmarc JR, et al. 1998. Am. J. Clin. Pathol. 109:682. (IHC)
3. Engleman EG, et al. 1981. Proc. Natl. Acad. Sci. USA 78:1791. (IHC, IP)
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310. (FC, IP)
5. Warnke R and Levy R. 1980. J. Histochem. Cytochem. 28:771. (IHC)