Alexa Fluor® 647 anti-human CD134 (OX40)
Description
CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4+ T cell tolerance.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
References
1. Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP)
2. Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC)
3. Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC)
4. Willett B, et al. 2007. J. Virol. 81:9665. (ELISA)
5. Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA)
6. Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed
7. Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)