Brilliant Violet 785™ anti-human CD3

Antibodies Single
Sony
SK7
Flow Cytometry
Mouse IgG1, κ
Human
2324205
$220.00

Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.

References

1. Kan EA, et al. 1983. J. Immunol. 131:536.
2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)