True-Nuclear™ Human Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD4 PE-Cy5/CD25 PE)

Cocktail
Sony
150D
Intracellular Staining for Flow Cytometry
N/A
Human
25 tests
2200135
$545.00

Description

T regulatory (Treg) cells are a subset of T lymphocytes which is characterized by CD4+/CD25+/FOXP3+. These naturally occurring Treg cells originate in the thymus, and comprise 2-10% of peripheral CD4+ T cells. It has been shown that Treg cells are able to inhibit T cells proliferation and cytokine production and play critical roles in preventing autoimmunity as well as in controlling tumor immunity and transplantation tolerance. Impaired Treg function or Treg cell deficiency will develop variety of autoimmune diseases, while higher frequency of Treg cells will cause hypo-immune response to pathogens.


's True-NuclearHuman Treg Flow™ Kit is designed and formulated specifically for immunofluroscence staining and flow cytometric analysis of human Treg cells in a mixed lymphocyte population. This kit is composed of fluorochrome conjugated anti-human CD4, CD25, FOXP3 antibodies, and the critical buffers. It is easy to use for identification of Treg cells.

References

1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
2. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol
3. Yang ZZ, et al. 2006. Blood 107:3639.
4. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659.
5. Groh V, et al. 2006. Nature Immunology 7:755.
6. Lewkowicz P, et al. 2006 J. Immunol. 177:7155.
7. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023.
8. Bamias G, et al. 2007. J. Immunol. 178:1809.
9. Valencia X, et al. 2007. J. Immunol. 178:2579. PubMed
10. Davidson TS, et al. 2007. J. Immunol. 178:4022.
11. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249.
12. Jaffar Z, et al. 2007. J. Immunol. 179:6193.
13. Müller M, et al. 2007. J. Immunol. 179:2774.
14. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed
15.Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed
16. Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed
17. Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381
18. Klunker S, et al. 2009. J. Exp Med. PubMed
19. Haque A, et al. 2010. J. Immunol. 184:2583. PubMed
20. Li S, et al. 2014. Virus Res. 180:84. PubMed
21. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed
22. Thauland TH, et al. 2014. J Immunol. 193:5894. PubMed