Alexa Fluor® 700 anti-human HLA-A,B,C

Antibodies Single
Sony
W6/32
Flow Cytometry
Mouse IgG2a, κ
Human
2157185
$121.00

Description

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Darrow TL, et al. 1989. J. Immunol. 142:3329.
2. Stern P, et al. 1987. J. Immunol. 138:1088.
3. Tran TM, et al. 2001. Immunogenetics 53:440.
4. Barbatis C, et al. 1981. Gut 22:985.
5. Ayyoub M, et al. 2004. Cancer Immunity 4:7.
6. DeFelice M, et al. 1990. Cell. Immunol. 126:420.
7. Fayen J, et al. 1998. Int. Immunol. 10:1347.
8. Turco MC, et al. 1988. J. Immunol. 141:2275.
9. Geppert TD, et al. 1989. J. Immunol. 142:3763.
10. Wooden SL, et al. 2005. J. Immunol. 175:1383.
11. Nagano M, et al. 2007. Blood 110:151.
12. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
13. Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
14. Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
15. Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
16. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
17. Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
18. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
19. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
20. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed