Brilliant Violet 785™ anti-human CD154

Antibodies Single
Sony
24-31
Flow Cytometry
Mouse IgG1, κ
Human
2154205
$226.00

Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.

References

1. Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF)
2. Rushworth SA, et al. 2002. Transplantation 73:635. (Block)
3. Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF)
4. Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block)
5. Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed
6. Kuchen S, et al. 2007. J. Immunol. 179:5886.
7. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed