Description

CDw137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CDw137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CDw137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

References

1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)