APC/Cy7 anti-human Perforin

Antibodies Single
Sony
dG9
Flow Cytometry
Mouse IgG2b, κ
Human
Purified granules from the human lymphoma cell line
2140640
$376.00

Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
3. Watanabe N, et al. 1997. Blood 90:3662.
4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
6. Chen H, et al. 2005. J. Immunol. 175:591.
7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
8. Wood SM, et al. 2009. Blood 114:4117. PubMed
9. Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.