Brilliant Violet 711™ anti-human CD123

Antibodies Single
Sony
6H6
Flow Cytometry
Mouse IgG1, κ
Human
Human IL-3Rα transfected COS cells.
2130145
$226.00

Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.

References

1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
2. Herling M, et al. 2003. Blood 101:5007. (IHC)
3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed 
7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)