Alexa Fluor® 488 anti-human CD42b
Description
CD42b is a 145 kD glycoprotein known as gpIbα. It is covalently bonded to CD42c to form GPIb. CD42b is expressed on platelets and megakaryocytes. CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for von Willibrand factor and thrombin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
References
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Takahashi R, et al. 1999. Blood. 93:1951.
3. Saggu G, et al. 2013. J. Immunol. 190:6457. PubMed
4. Meyer Dos Santos S, et al. 2011. Blood. 117:4999. (Block) PubMed
5. Vettore S, et al. 2008. Haematologica. 93:1743.