Alexa Fluor® 594 anti-human CD4
Description
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 2.5-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
References
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
6. Lan RY, et al. 2006. Hepatology 43:729.
7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)