Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8⁺ T cells.

Formulation

Recommended Usage

Dilute 18 microL of HRP anti-human β2-microglobulin antibody in 12 ml of assay dilution buffer. This is sufficient for one 96 well plate.

References

1. Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed
2. Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed
3. Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.