Description

The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, NK cells, and a subset of lymphocytes. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin α_M and α_X chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
2. Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC)
3. Robinson AP, et al. 1986. Immunology 57:239. (Block)
4. Issekutz SE, et al. 1992. Immunology 76:655. (Block)
5. Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
6. Thomas RA. et al. 2009. J Pharmacol Toxicol Methods. 60:263. PubMed
7. Takeda Y, et al. 2011. Immunobiology. 216:1094. PubMed
8. Furuhashi K, et al. 2013. J Am Soc Nephrol. PubMed
9. Cheng HY, et al. 2013. Cytotherapy. 1465:608. PubMed