Description

Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 microg per million cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Trimble L, et al. 1998. Blood 91:585.
2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
4. Chen H, et al. 2005. J. Immunol. 175:591.