Biotin anti-mouse CD284 (TLR4)

Antibodies Single
Sony
SA15-21
Flow Cytometry
Rat IgG2a, κ
Mouse
LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
100 µg
1327045
$214.00

Description

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells, and T cells. TLR4 has been recognized as critical for host recognition of LPS in conjunction with MD2.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
2. Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
3. Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
4. Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)