APC/Cy7 anti-mouse CD172a (SIRPα)

Antibodies Single
Sony
P84
Flow Cytometry
Rat IgG1, κ
Mouse
Mouse brain membrane protein
1320090
$352.00

Description

CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
2. Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
3. Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
4. Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)