Alexa Fluor® 700 anti-mouse CD41
Description
CD41, also known as integrin α2b and GPIIb, is a transmembrane glycoprotein that is expressed by platelets and megakaryocytes. It was reported that CD41 is also expressed on hematopoietic progenitors. CD41 associates with CD61 (integrin β3) to form complexes that interact with fibrinogen, fibronectin, von Willebrand factor, and thrombin. CD41 is required for platelet adhesion and aggregation. Defect of CD41 leads to disorders of coagulation.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
References
1. Nieswandt B, et al. 1999. Blood 94:684.
2. Teeling JL, et al. 2001. Blood 98:1095.
3. Bertrand JY, et al. 2005. P. Natl. Acad. Sci. USA 102:134.
4. Nocito A, et al. 2007. Hepatology 45:369. (Deplete)
5. Sullivan BP, et al. 2010. Toxicol. Sci. 115:286. (Deplete) PubMed
6. van der Heyde HC, et al. 2005. Blood 105:1956. (FA)
7. Marjon KD, et al. 2009. J. Immunol. 182:1397. (Deplete)