Brilliant Violet 510™ anti-mouse CD90.2

Antibodies Single
Sony
30-H12
Flow Cytometry
Rat IgG2b, κ
Mouse
Mouse thymus or spleen
50 µg
1126675
$290.00

Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

References

1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
6. Hardy B, et al. 2005. Int. Immunol. 17:615.
7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed