FluoroFix™ Buffer
Description
FluoroFix™ Buffer is a ready-to-use buffer, specially formulated for fixation of immunofluorescence stained cells, optimized to stabilize tandem dyes. It can be used as the final resuspension of the cell pellet in immunofluorescence staining procedures. FluoroFix™ Buffer is provided as 100 mL.This quantity is sufficient for 200 tests.
Recommended Usage
After final wash in the staining procedures , discard supernatant and resuspend the cell pellet in 0.5 ml/tube FluoroFix™ Buffer and analyzed with flow cytometry. If the samples can not be analyzed within 4 hours, resuspend the cell pellet in 0.5 ml/tube FluoroFix™ Buffer and incubate in the dark for 30 minutes at room temperature. Wash 1X with Cell Staining Buffer (cat# 420201) and resuspend in 0.5ml/tube Cell Staining Buffer, cover the tubes.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.