Alexa Fluor® 647 anti-human HLA-DR, DP, DQ
Description
HLA-DR, HLA-DP, and HLA-DQ are heterodimeric cell surface glycoproteins comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
References
1. Pawelec G, et al. 1985. Hum. Immunol. 12:165.
2. Shaw S, et al. 1985. Hum. Immunol. 12:191.
3. Ziegler A, et al. 1986. Immunobiology 171:77.
4. Pawelec G, et al. 1986. Hum. Immunol. 17:343. (Block)
5. Dai Z, et al. 2009. J. Exp. Med. 206:793. (Block)
6. Pawelec G, et al. 1988. J. Exp. Med. 167:243. (IP)