FITC anti-human HLA-DR

Antibodies Single
Sony
Tü36
Flow Cytometry
Mouse IgG2b, κ
Human
Human PBL
2408020
$249.00

Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Pawelec G, et al. 1985. Hum. Immunol. 12:165. (FC)
2. Shaw S, et al. 1985. Hum. Immunol. 12:191. (FC)
3. Ziegler A, et al. 1986. Immunobiology. 171:77. (FC)
4. Cebulla CM, et al. 2002. J. Immunol. 169:167. (WB, FC, IP)
5. Khaw LT, et al. 2013. PLOS One. 8:e69521. (Block)