PE anti-human IL-4

Antibodies Single
Sony
MP4-25D2
Flow Cytometry
Rat IgG1, κ
Human
CHO-expressed, recombinant human IL-4
3104040
$98.00

Description

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
microg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.
Cat. No. 500808: For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood.
Cat. No. 500810: For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume.
It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
7. Raqib R, et al. 1995. Infect. Immun. 63:289.
8. Andersson J, et al. 1994. Immunology 83:16.
9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
10. Kubota M, et al. 1997. J. Immunol. 158:5321.
11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
12. Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed