Presented by Select Science

Autoimmunity to citrullinated autoantigens forms a critical component of disease pathogenesis in rheumatoid arthritis (RA). Recently, several citrullinated antigen-specific CD4+ T cells have been described. We present a pipeline for TCR repertoire analysis of antigen-specific CD4+ T cells from RA patients for multiple citrulline specificities using cell expansion induced by an in vitro peptide challenge.

Join this webinar to learn how single-cell sorting and index analysis, combined with integrated TCR sequencing and demultiplexing the TCR repertoire, were performed. We will discuss a custom workflow that enables linking flow cytometry index-sorting data from multiple platforms with a TCR repertoire profile of antigen-specific CD4+ T cells. The output from the pipeline includes retrieval of index-sort information, normalized fluorescence intensities across patients and platforms, as well as the information combined with expanding TCR clones. Overall, this workflow results in a comprehensive and easy approach to generate and analyze multidimensional data for immune cell profiling.

In this webinar, you will also:

  • Review techniques used for profiling the TCR repertoire of antigen-specific CD4+ T cells from RA patients
  • Learn how data sets generated on different flow cytometry platforms can be integrated with single-cell RNA Seq analysis to generate multidimensional data


Who should attend

This webinar will provide insights to researchers who want to explore approaches that integrate different single-cell analysis technologies for immune cell profiling and want to learn how large data sets generated across various platforms can be combined to study multidimensional biology.



Photo of Dr. Ravi KumarDr. Ravi Kumar
Researcher, Karolinska Institute

Dr Ravi Kumar, PhD is currently a Postdoctoral Fellow affiliated with the Division of Rheumatology, Department of Medicine, Karolinska Institutet, Sweden. His work in Prof. Vivianne Malmström’s laboratory at Center for Molecular Medicine focuses on development of high throughput methods for identifying autoreactive T cells by T-cell receptor sequencing. The lab uses various tools including HLA class II tetramers and activation induced marker expression systems, after in-vitro cultures to identify functional antigen-specific CD4 + T cells using multicolor flow cytometry and study their TCR repertoire in rheumatoid arthritis and ANCA associated vasculitis.

Faris Naji
CEO, Tercen

Faris Naji is currently co-founder and CEO of Tercen, a cloud-based analytics company developing solutions to bridge the analysis gap between the wet-lab and dry-lab. He holds degrees in computer science and mathematics, with over 20 years of experience in biotech companies that generate large amounts of molecular measurements and has seen first-hand the issues it brings.