Deciphering somatic mosaicism in healthy tissues and clonal diversity in tumors necessitates single-cell analysis. High-quality genomic and transcriptomic data at the single-cell level depends on the efficient isolation of individual cells, coupled with robust amplification techniques upstream of sequencing.

In this webinar, we present a methodology using the SH800 Cell Sorter and ResolveOME™ multi-omic single-cell/single-nucleus amplification for comprehensive genomic analysis. Studies using this combinatorial approach to analyze the transcriptional states and mutations in invasive breast cancer cells and to study copy number variation (CNV) profiles from primary neurons, murine brain, and cultured cell lines will be presented.

Overall, the approach of integrating cell and nuclei sorting with multi-omic analysis enables the critical pairing of genotype with phenotype and provides critical insights into tumor evolution and somatic mosaicism in normal tissues.

Key Learning Objectives:

  • Explore how microfluidics-based cell sorting with SH800 can be used to purify epithelial cells from immune enriched breast cancer samples and sort nuclei from primary cells and cell lines
  • Learn how the ResolveOME™ technology can be used for robust genome and transcriptome amplification that can be used for next generation sequencing and bioinformatic analysis with BaseJumper® computational tools
  • Uncover crucial understandings regarding tumor evolution and somatic mosaicism in normal tissues, thereby enhancing comprehension of cancer heterogeneity and progression

 

Who should attend

Researchers, scientists in oncology, cancer biology, and genomics, along with biomedical professionals and clinicians, interested in tumor heterogeneity, single-cell analysis techniques, and cancer progression mechanisms.

 

Speaker

Portrait of Jon S. ZawistowskiJon S. Zawistowski, PhD
Senior Director of R&D
BioSkryb Genomics, Durham, NC

Jon received his PhD from Duke University Medical Center, and after postdoctoral training and positions in academia and the biotechnology sector, joined BioSkryb Genomics, Inc. He currently directs their R&D team, advancing core single-cell genomic amplification chemistry and extending it to enable multi-omic insights from individual cells, with a focus on primary breast cancer.