Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
5. Zan H, et al. 2005. EMBO J. 24:3757.
6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
8. Lei F, et al. 2013. J Vis Exp. 60:3986. PubMed.