Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 microg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 microg per 10⁶ cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)